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KMID : 0380420150390030093
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Journal of Prventive Veterinary Medicine
2015 Volume.39 No. 3 p.93 ~ p.100
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Anti-inflammatory effects of equine adipose-derived mesenchymal stem cells for bone fracture in thoroughbred racehorses
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Lee Gyeong-Been
Lee Jienny
Sohn Yong-Woo
Gu Na-Yeon
Kim Hee-Ryang
Byeon Jeong-Su
Jeon Hyung-Seon
Jang Jong-Duck
Yang Young-Jin
Cho In-Soo
Cha Sang-Ho
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Abstract
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Bone fractures are most often seen in racetrack horses because of the high level of intensity in racing. These issues are the main cause of decreased performance in racehorses. Mesenchymal stem cells (MSCs) have been explored to improve intra-articular therapy in racehorses. MSCs are essential for the repair and regeneration of damaged tissues. In this study, the effect of intra-articular injection of MSCs in racehorses was investigated. Before accessing the MSC therapy, synovial fluids were obtained from the fracture site of racehorses, and adipose tissue was collected for MSC isolation. Using the MSC specific marker, adipose tissue-derived MSCs were identified. The racehorses received intra-articular injection of autologous MSCs (or allogeneic) (3 × 107 cells/3 mL). After 1 or 2 weeks, synovial fluids were collected from racehorses. To test the effect of MSC injection using ELISA, we analyzed inflammatory factors from the untreated samples compared to MSC-treated samples of racehorses. The level of pro-inflammatory factors (interleukin-1β and prostaglandin E2) was significantly decreased in synovial fluids of MSC-injected racehorses, compared to before accessing the MSC therapy, whereas, the level of anti-inflammatory factor (interleukin-10) was higher than prior to accessing the MSC therapy. Further studies are needed to investigate the anti-inflammatory mechanism of MSC in racehorses.
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KEYWORD
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Equine adipose tissue, mesenchymal stem cells, bone fracture, cell therapy, anti-inflammatory factor
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